Tissue grinders disperse and disrupt small tissue samples by being repeatedly pressed or sqeezed between two hard surfaces. Usually, one surface is concave shaped (the mortar) and other convex (the pestle). The motar can also be test tube-shaped, in which case a tight-fitting pestle having a clearance of 0.1 - 0.2 mm is used. Tissue is repeatedly sqeezed through this mortar-pestle clearance.
Soft tissue samples with high water content (example; liver, muscle, fruits) frozen to and kept at dry ice- or liquid nitrogen temperatures are very easily fractured into powder using the crushing action of a motar and pestle. This special process is called cryogrinding.
Another catagory of mechanical homogenizer that also operates, at least in part, by similiar hard surface to hard surface grinding forces are beadbeaters (aka, bead mills).
The BioSqueezer is used to snap freeze (quickly freeze) 10-100 mg of soft animal and human tissue. Fresh tissue is compressed into a thin disc about the size of a small coin under the pressure of a stainless steel clamp head that has been pre-cooled in liquid nitrogen or dry ice. Freezing occurs in a few milliseconds, thus giving a snapshot of metabolic events in the cells. The hard frozen disc is easily fragmented or ground without thawing for subsequent extraction of labile nucleic acids, proteins and intracellular metabolite products.
The Cryo-Cup Grinder is a mortar and pestle specifically designed to pulverize and powder plant and animal tissue samples at liquid nitrogen temperatures. It gives superior results compared to conventional ceramic mortar-pestles.
Cat. No. 206, Cryo-cup Grinder, complete, includes two different size polypropylene pestle balls. See below for extra cups (mortars) and pestle balls.
Cat. No. 206C, Extra s.s. mortar with insulated cup. (Pestle not included)
Cat. No. 206PP138, Polypropylene ball, 1 3/8 inch dia.
Cat. No. 206PP178, Polypropylene ball, 1 7/8 inch dia.
Cat. No. 206SS138, Stainless steel ball, 1 3/8 inch dia.
Cat. No. 206SS178, Stainless steel ball, 1 7/8 inch dia.
The CryogenicTissueGrinder is a high speed blade mill that grinds 0.5-10 grams of plant, animal and human tissue with high water content to a fine powder in the presence of dry ice. The tissue becomes brittle at solid CO2 temperatures. A sample hit with a hammer (or in the case of the CTG111, a spinning blade) fragments into tiny pieces in a matter of seconds.
Cat. No. CTG111, 120 volts
A beautiful, hand-made mortar & pestle sculptured out of onyx stone.
The NEW SpiralPestle™ and 1.5 ml Microtube grinder is an significant improvement over conventional smooth-walled polypropylene pestle and tube combinations. It is used to homogenize culture cells and small samples (<100 mg wet weight) of soft tissue such as liver, brain, and muscle. It is also routinely used to disperse centrifugal pellets, precipitates, and other solids.
Cat. No. 1017, SpiralPestles™ and 1.5 ml microvials (100 count) and sample of Spiral Grinding Beads.
Cat. No. 1017MC, Above Cat. No. 1017 plus a BioVortexer™ motor (Cat. No. 1083).
Cat. No. 1017GB, SpiralPestle Grinding Beads. Glass beads for pestle and microvial grinders, 50 cc bottle with pour spout.
All stainless steel Potter-Elvehjem style tubular mortar and pestle. Superb temperature control during use, shatter-free, easy to clean.