Lever-type Tissue Press
The Lever Tissue Press is a modified garlic press enhanced by addition of a silicone-rubber gasket to the bottom of the lever press piston and the inclusion of an extra "can" to facilitate processing of multiple samples.
The Lever Tissue Press is used as a mincing step for isolation of single cells, the extraction of intracellular components such as nuclei and mitochondria and as a preliminary step in the preparation of a total cell lysate. Unlike many other tissue dispersion/blending/homogenizing devices, water or buffer is NOT added in its operation.
Special App - Disaggregation of tissue for primary culture: Isolation of cells from solid tissue samples commonly involves digestion of extracellular matrix with proteolytic enzymes. The first step in most protocols call for manually chopping the tissue into little pieces with a scissors or scalpel in order to increase access of the digestive enzymes to tissue surfaces. The Lever Tissue Press eliminates this tedious manual chopping and delivers a significant improvement by producing much smaller tissue pieces than chopping. Incubation time needed for enzyme-assisted tissue disaggregation is significantly reduced and diffusion of nutrients and gasses to the tissue mass is increased, thus optimizing cell viability during the incubation period.
For an excellent review of Enzymatic Disaggregation of Tissue Samples see http://www.worthington-biochem.com/tissuedissociation/default.html .
Retract the piston of the Lever Tissue Press from its stainless steel can. Add 1 to 10 g of fresh tissue to the cavity of the stainless steel can and swing the piston into position. Grip the levers together, thus forcing the piston down the press can or barrel. The dispersed tissue protruding from the bottom of the can is removed with the swipe of a single edge razor blade or scapel.
Note: Tissue must be "soft", relatively non-fibrous and have high water content. Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers. If the tissue is fatty or partially fibrous trim off the tough or fatty parts before pressing. Significant fibrous content in tissue samples interferes with the passage of the tissue through the press and lowers the yield of dispersed cell material. Unacceptible tissues are skin, hide, connective tissue, woody or fibrous plant stems, many dry seeds and tissues. Rehydration of dried samples may make them amenable.