Selected Applications Using the Mini-Beadbeater
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Extraction from Soil & Wastes
Small-scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil, C.R. Kuske, et. al., Appl. Environ. Microbiology, Vol.64, p.2463-2472 (1998). "Only the MiniBeadbeater was effective for DNA extraction from Bacillus globigii (B. subtilis subsp. niger) endospores or Fusarium moniliforme conidia"
Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-based Microbial Community Composition, R.I. Griffiths et. al., Appl. Environ. Microbiology, Vol 66, p.5488-5491 (2000)
Simultaneous DNA/RNA Extraction from Soil, a protocol based on a modified method of Griffiths et. al.(2000), from Eoin Brodie, April 2003. This protocol uses a Fast-Prep™ machine. For comparable results with other beadbeater-type machines use the maximum available speed setting.
A Strategy for Optimizing Quality and Quantity of DNA Extracted from Soil, Helmut Burgmann et al, J Micrrobiological Methods, Vol 45, p. 7-20 (2001). Examines the effect of varying beadbeating time, speed, volume and temperature of extraction media, and amount and type of beads on the yield and quality of DNA from several soil types.
Improved Extraction of PCR-quality Community DNA from Digesta and Fecal Samples, Zhongtang Yu and Mark Morrison, BioTechniques, Vol 36, p.808-813 (2004). Content: Beadbeating gives "1.5 to 6 fold increase in DNA yield compared to other methods.
Comparative Analysis of Fecal DNA Extraction Methods with Phylogenetic Microarray: Effective Recovery of Bacterial and Archaeal DNA using Mechnical Cell Lysis, Anne Solonen et al, J Microbiological Methods, Vol 81, p.127-134 (2010). Content: Four widely used methods are compared. The DNA yields varied up to 35 fold. A modified protocol of Yu and Morrison was the best.
Skim Milk Drastically Improves the Efficiency of DNA Extraction from Andisol, a Volcanic Ash Soil, Yuko Takada Hoshino and Naoyuki Matsumoto, Japan Agri Research Quart, Vol 39-4, 247-252 (2005) Content: 40 mg skim milk powder/gram soil sample was added as an adsorption competitor. Does not interfere with PCR procedures.
Virus & Spores
A Field Investigation of Bacillus anthracis Contamination of USDA and Other Washington, DC Buildings during the Antrax Attack of October 2001, James A. Higgins, et. al., Appl. Environ. Microbiology, Vol.69, p.593-599 (2003)
Extraction and Analysis of Cortisol from Human and Monkey Hair, Jerrold Meyer, Melinda Novak, Amanda Hamel, and Kendra Rosenberg, J Vis Exp. 2014; (83): 50882 (2014). To a 2 ml microcentrifuge tube add 60 mg washed hair, three 3.2 mm chrome steel beads and grind for at 2-3 min in a MiniBeadbeater. Includes a video link showing protocol details.
Pathogens in Plants