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BioHomogenizer

General Procedures

  1. Chop or cut tissue into small pieces (approximately 1mm in length) using a scalpel, safety razor blade or scissors. Especially relevant when isolation of nucleic acids is the objective, if your sample is already stored prefrozen do not thaw the tissue sample.  Instead, cryopulverize the sample (see BioPulverizer).
  2. Place tissue in homogenizing vessel containing the equivalent of 3 to 10 tissue-volumes of homogenizing medium.
  3. Lower the the probe into tissue suspension, turn on the BioHomogenizer, and operate for 5 seconds to 2 minutes using an up-and-down dabbing motion. Do not immerse the probe more than one-half its length into the homogenization solution. Use lower speeds for subcellular particle preparation and maximum motor speed for biochemical or macromolecule extractions. If desired, the homogenizing vessel can be placed in an ice bath during homogenization. The BioHomogenizer can be stand-mounted using a tri-clamp and ring-stand. This is convenient when processing numerous samples.
  4. To avoid splashing, turn off the Tissue-Tearor before withdrawing from the homogenizing vessel.
  5. Place the BH upright in a stand when not being used so that liquids wisthin the probe can drain out. Do not place it on its side.

Cleaning:

Prompt cleaning of the BioHomogenizer is important. Many biological materials and extraction media are surprisingly corrosive, even if the probe is stainless steel. If  biomaterial gets into the upper part of the probe and into the motor, the lower motor bearing will be destroyed. Between repetitive sample processing, the unit can be superficially cleaned by briefly operating the TT in a vessel containing water or detergent. At the end of the work day, unscrew the outer part (stator) by turning clockwise (it's left-hand threaded) and wash in detergent, dry the parts and reassemble the stator.  If desired, the lower parts of the probe (stator and rotor) can be removed from the motor unit and autoclaved.  The rest of the probe (shaft) cannot be autoclaved.  Instead, wipe it down with a 1 to 10 dilution of household bleach followed with a sterile water. rinse

Maintenance:

From time to time remove the stator and rotor, hold the BH up-side-down and add a  single drop of mineral oil to the probe shaft.  Do not use a petroleum-based lubricant.  Run the BH in this position for 30 seconds. Wipe away excess lubricant.  Reattach the rotor and stator to the shaft.