Bio Spec Industries

Go to content

info@biospec.com
800.617.3363 or 918.336.3363

Tissue Dispersers

Tissue dispersers are often the starting point when isolating individual cells from samples of tissue. The action of these mechanical devices alone, sometimes in combination with enzymatic digestion of the intracellular matrix, is gentle enough to disperse fresh tissue into individual, viable cells.

As might be expected, no tissue disperser is perfect. Depending on the method used, the dispersion process yields some cells that are no longer viable and there is always a proportion of tissue remaining as small, multicellular aggregates.

BioChopper Kit

BioChopper Kit

Our BioChopper Kit provides two simple tools to aid in preparing your tissue samples for homogenation.

Learn More »

DuelingSyringes™ Tissue Disperser

DuelingSyringes™ Tissue Disperser

A low cost, disposable, tissue disperser for up to 100 mg samples of soft tissue (e.g., liver, brain and muscle).  The Dueling Syringes™ tissue disperser is a significant improvement over the classic "syringe-needle-syringe" method of tissue dispersion.  The needle is replaced with an embedded 500 micron stainless steel screen inside a 1 ml polycarbonate syringe.  Manual passage of the sample back and forth through the screen is fast, gentle and efficient.  Sold as a pack of five pairs of syringes.

Learn More »

Lever Tissue Press

Lever Tissue Press

The Lever Tissue Press is a top-of-the-line stainless steel garlic press upgraded in performance by the addition of a silicone-rubber gasket on the lever press piston.The press efficiently macerates or mechanically disaggregates 5 to 50 grams of soft plant or animal tissue.  By 'soft' we mean fresh, nonfibrous tissue.  Acceptable examples are muscle, brain, liver, plant fruits, some plant roots and tubers.  An extra "can" is included to facilitate processing of multiple samples. 

Learn More »

MicroMincer

MicroMincer

Cat. No. 2941, MicroMincer laboratory press complete with a 10-pack of liner and seals
Cat. No. 294103, Extra liners and seals, 10 of each

Learn More »

Screw Tissue Press

Screw Tissue Press

<meta name='uuid' c><meta charset='utf-8'>

CELL LYSIS  The Screw Tissue Press is used to initially disperse soft animal tissue (e.g., muscle, kidney, spleen, liver) before using mechanical, detergent, or chemical-based methods to lyse the cells. Compared to manually chopping using scissors or a scalpel, much smaller tissue fragments are produced and the cell lysis that follows will be faster and more complete. Notably, the addition of buffer is not required to use the Screw Tissue Press. The dispersed tissue exits the Screw Tissue Press having a consistency much like liver paté or toothpaste.

SINGLE CELL PREPARATION  The Screw Press is also used in the preparation of viable single cells from soft animal tissue by enzymatic digestion of connective matrix.  Screw tissue Press dispersion delivers higher yields of viable cells by significantly reducing incubation time with proteases and enhancing the diffusion of essential nutrients and gases during the incubation with the enzymes.

Key Single-cell Preparation references:

Methods of Enzymatic Tissue Disaggregation, Enhanced Enzymatic Dispersion of Primary Cells from Tissue

A Rapid Method for the Preparation of Single‐cell Suspensions from Solid Tissues, N. P. Singh, Cytometry 31, 229–232, 1998, >>https://onlinelibrary.wiley.com/doi/epdf/10.1002/(SICI)1097-0320(19980301)31:3<229::AID-CYTO10>3.0.CO;2-T

Learn More »

SpiralPestle™ and MicroTube Homogenizer

SpiralPestle™ and MicroTube Homogenizer

 

The NEW SpiralPestle™ and 1.5 ml Microtube grinder is a significant improvement over traditional smooth-walled polypropylene pestle and tube combinations.  It is used to homogenize culture cells and small samples (<100 mg wet weight) of soft tissue such as liver, brain, and muscle.  It can also be used to disperse centrifugal pellets, precipitates, and other solids.

Cat. No. 1017, SpiralPestles™ with matching 1.5 ml microvials (100 count) and a sample of Spiral Grinding Beads.

Cat. No. 1017MC, BioVortexer™ Tissue Grinding Kit.  Includes BioVortexer motor drive, batteries, 100 SpiralPestles™ with matching 1.5 ml microvials and a sample of SpiralPestle™ Grinding Beads.

Cat. No. 1017GB, SpiralPestle™ Grinding Beads.  Glass beads for pestle and microvial grinders, 50 cc bottle with pour spout.

Learn More »

BioSpec Informer

Subscribe to our alerts for new product releases, special offers and educational opportunities.

* indicates required

Email Format