Tissue dispersers are the starting point when isolating individual cells from samples of tissue. The action of these mechanical devices alone, or sometimes in combination with enzymatic digestion of the intracellular matrix, is gentle enough to disperse fresh tissue into individual, viable cells.
As might be expected, no tissue disperser is totally efficient. Depending on the technique used, the dispersion process yields some cells that are no longer viable and a proportion of tissue will remain as small, multicellular aggregates.
Our BioChopper Kit provides two simple tools to aid in preparing your tissue samples for homogenation.
A low cost, disposable, tissue disperser for up to 100 mg samples of soft tissue (e.g., liver, brain and muscle). The Dueling Syringes™ tissue disperser is a significant improvement over the classic "syringe-needle-syringe" method of tissue dispersion. The needle is replaced with an embedded 500 micron stainless steel screen inside a 1 ml polycarbonate syringe. Manual passage of the sample back and forth through the screen is fast, gentle and efficient. Sold as a pack of five pairs of syringes.
The Lever Tissue Press is a top-of-the-line stainless steel garlic press improved by the addition of a silicone-rubber gasket on the lever press piston.The press efficiently macerates or mechanically disaggregates 5 to 50 grams of fresh, soft plant or animal tissue. By 'soft' we mean nonfibrous tissue. Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers. An extra "can" is included to facilitate processing of multiple samples.
Cat. No. 2941, MicroMincer laboratory press complete with a 10-pack of liner and seals
Cat. No. 294103, Extra liners and seals, 10 of each
The Screw Tissue Press is used as an initial tissue dispersion step before lysing fresh tissue with a mechanical homogenizer (ultrasonicator, beadbeater, rotor-stator, etc). Chopping tissue into pieces with a scissors or scalpel is eliminated, much smaller tissue fragments are produced. Unlike other tissue dispersion/blending devices, no buffer or media is added during its operation. The dispersed tissue exits out of the end of the Screw Tissue Press having a consistancy much like liver pate or tooth paste.
The Screw Press also is used to prepare viable single cells by proteolytic digestion of extracellular connective matrix. The benefits of an initial Screw Press disaggregation of tissue is reduced incubation times with the digestive enzymes and increased diffusion of critical nutrients and gases into tissue cells during the incubation period. The net result is higher yields of viable cells. [For an excellent review of the Methods of Enzymatic Tissue Disaggregation see http://www.worthington-biochem.com/tissuedissociation/default.html ].
Note that tissue processed in this press must be "soft". That is, relatively non-fibrous and have high water content. Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers.
The NEW SpiralPestle™ and 1.5 ml Microtube grinder is an significant improvement over traditional smooth-walled polypropylene pestle and tube combinations. It is used to homogenize culture cells and small samples (<100 mg wet weight) of soft tissue such as liver, brain, and muscle. It can also be used to disperse centrifugal pellets, precipitates, and other solids.
Cat. No. 1017, SpiralPestles™ with matching 1.5 ml microvials (100 count) and a sample of Spiral Grinding Beads.
Cat. No. 1017MC, BioVortexer™ Tissue Grinding Kit. Includes BioVortexer motor drive, batteries, 100 SpiralPestles™ with matching 1.5 ml microvials and a sample of SpiralPestle™ Grinding Beads.
Cat. No. 1017GB, SpiralPestle™ Grinding Beads. Glass beads for pestle and microvial grinders, 50 cc bottle with pour spout.