Tissue dispersers are the starting point when isolating individual cells from samples of tissue. The action of these mechanical devices alone, or sometimes in combination with enzymatic digestion of the intracellular matrix, is gentle enough to disperse fresh tissue into individual, viable cells.
As might be expected, these dispersers are not totally efficient. The dispersion process also yields some cells that are no longer viable and a significant proportion of the dispersed tissue remains as small cell aggregates.
A low cost, disposable, tissue disperser for up to 100 mg samples of soft tissue (e.g., liver, brain and muscle). The Dueling Syringes™ tissue disperser is a significant improvement over the classic "syringe-needle-syringe" method of tissue dispersion. The needle is replaced with an embedded 500 micron stainless steel screen inside a 1 ml polycarbonate syringe. Manual passage of the sample back and forth through the screen is fast, gentle and efficient. Sold as a pack of five pairs of syringes.
The Lever Tissue Press is used as a mincing step for isolation of single cells or the extraction of intracellular components such as nuclei and mitochondria. Unlike other tissue dispersion/blending devices, no added water or buffer is required in its operation. See Narendra P Singh, et. al. for preparation of viable single cell suspensions [DNA double-stranded breaks in mouse kidney cells with age, Narendra P Singh et. al., Biogerontology 2, 261-270 (2001)].
The Lever Tissue Press, a modified garlic press, enhanced by addition of a silicone-rubber gasket to the bottom of the lever press piston and the inclusion of an extra "can" to facilitate processing of multiple samples.
Cat. No. 2941, MicroMincer laboratory press complete with 10 pack of liner/seals
Cat. No. 294103, Ten pack of extra liner/seals
The Screw Tissue Press is a classic screw press, designed to efficiently macerate or mechanically disaggregate 5 to 50 grams of fresh, soft plant or animal tissue. By 'soft' we mean nonfibrous tissue like brain, liver, muscle, potato, callus, etc. Tough or fibrous tissues like leaves or skin will not pass through the stainless steel screen.