The Mini-Beadbeater-96 is high-energy, high-throughput cell disrupter. It can process one 2 ml deep well micro plate (96 samples), two 1 ml deep well microplates (192 samples) or, using a included 45 capacity polypropylene microvial rack, 0.6, 1.5 or 2 ml microvials. In a busy day thousands of samples can be processed.
Cat. No. 1001, Mini-Beadbeater-96, 115 volt.
Cat. No. 1001EUR, Mini-Beadbeater-96, 230 volt.
Beads and vials vary with the application and should be ordered separately. For selecting Beads and Vials see their links in Browse box in the left margin.
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The MiniBeadbeater-96 disrupts cells and tissue by violently shaking a sample inside a classic 2 ml microvial or standard deep well microplate partially filled with tiny ceramic or steel beads. Complete cell disruption is achieved in 1 to 3 minutes of beadbeating. A common application is nucleic acid extraction. Up to 400 mg of tissue is added to a vial containing glass or ceramic beads and a nucleic acid extraction solution...the later often being purchased as part of a nucleic acid extraction kit. The synergy of simultaneous mechanical cell disruption and nucleic acid extraction in a chaotropic solution gives very high yields of high quality RNA or DNA. Most protocols developed using smaller capacity models of the MBB are transferable with little if any modification.
The MiniBeadbeater can also be used for dry grinding. In this application, ceramic or steel beads are added to to vials containing hard, dry samples such as hair, bone, teeth, seeds and minerals. Softer materials such as biological tissue, rubber or plastics can be powdered by first pre-freezing the sample to liq N2 temperatures (called Cryo-grinding). Powdered material can be safely dissolved in an organic solvent in BioSpec's XXTuff microvials, our stainless steel microvials or Porvair's reinforced microplates, simplifying recovery of organic analytes. A solid aluminum vial holder can be utilized to maintain cryogenic temperatures during beadbeating (see the Accessories, below).
Selected Applications Using the Mini-Beadbeater
Helpful Advice when Buying a Commercial High Through-put Cell Disrupter
OPERATING INSTRUCTIONS: See http://www.biospec.com/instructions/minibeadbeater_96/.
The MiniBeadbeater-96 is powered with a huge 1 hp induction motor. No other beadbeater on the market offers higher shaking energies for cell disruption. Power requirements for the 115 VAC MBB-96 is 20 amps, and for the 230 VAC machine it is 10 amps.
- Sample Capacity: Holds one 2 ml deep-well microplate or two 1 ml deep-well microplates. Also included is a microplate shaped vial holder wich can hold 4 to 45 microvials (0.5, 1.5 & 2.0 ml) each containing up to 400 mg (wet weight) of biomaterial.
- Vial displacement distance or Throw: 1.25 in. (3.2 cm).
- Shaking Speed: Variable speed. 1400 rpm to 2400 rpm (40 oscillations/second). Disruption efficiency has been engineered for equivalency with other MiniBeadbeater models having smaller vial capacities - thus developed protocols are interchangeable. Its "calculated M/sec performance" value is greater than competitive beadbeater-type cell disrupters on the market.
- 3-D shaking pattern: Vial contents shake from the top to bottom of the vial or plate in a compressed figure-8 pattern. Vials and microplates are held in a near-horizontal orientation rather than the less efficient vertical position. This exclusive shaking pattern assures complete cell disruption in the shortest possible time. No reorientation of plates in mid-cycle is required.
- Timer: 0 - 5 minutes with automatic reset.
- Recycle delay time: No motor cool-down time is required during or between sample runs.
- Dimensions: Width: 16 in, Depth: 24 in, Height: 18 in, 120 pounds
- Maintenance: None
- Warranty: Two years.
IMPORTANT FEATURES AND CAPABILITIES
► COOLING. The Mini-BeadBeater-96, like all high-energy, bead mill cell disrupters, can heat up a sample from the collisional friction of the beads...about 10 deg for each minute of beadbeating. An important accessory for the MBB-96 is a 48 capacity 2 ml microvial holder and a 2 tube capacity 50 ml tube holder, both machined out of solid aluminum. Cooled beforehand in a deep freezer or with liquid nitrogen, the mass of the aluminum vial holder and its excellent heat transfer properties maintain samples at their initial low temperature during an entire beadbeating run...something that some competitor's bead mills offering flowing cold air accessories fail to accomplish. Cold air is not an efficient media for the rapid removal of heat. For the same reason, operating a bead mill cell disruptor in a cold room offers little regulation of heat buildup during beadbeating.
Temperature control must be addressed when using a high-throughput bead mill cell disrupter for the isolation of proteins, membranes or organelles. But this is not always the case. Strict temperature control is not a concern when cells or tissue are being disrupted in the presence of DNA or RNA extraction media.
► ”DRY” GRINDING. The Mini-BeadBeater-96 can be used to powder most dried plant and animal tissues and fresh tissues pre-chilled to liquid nitrogen temperatures. The exceptions are woody tissue and for tissue having high lipid or oil content.
When dry- or cryo-grinding plant or animal tissue use our stainless steel microvials with silicone rubber caps, special XXTuff microvials or special Porvair Sciences deep well microplates. Common plastic microvials and deep well plates will break when dry grinding with steel beads.
To the tubes or wells add either one 6.3 mm diameter chrome-steel bead, two 6.3 mm diameter glass beads or three 3.2 mm diameter chrome-steel beads and Beadbeat for 5-30 seconds. Note that steel beads can be used in common plastic vials and common deep well microvials provided that the vial is completely filled with aqueous media (i.e., wet milling).
► DEEP-WELL MICROPLATES. When using deep well microplates possible leakage and subsequent cross contamination between wells is a concern. While the entire mat surface area is evenly clamped to the microplate during beadbeating in the Mini-BeadBeater-96, the user must first thoroughly press the mat onto the microplate during the initial sealing process. Rollers and mat presses are available to help in this task (see accessories listed below). The best mats are made of EVA or silicone rubber. Mats made of other rubber-like polymers or adhesive- or heat-bonded films - may not prevent leaking during intense shaking in the Mini-BeadBeater-96.
► SPEED CONTROL. The Mini-BeadBeater-96 features digital variable speed control presets between 1400 rpm to 2400 rpm and three programmable time presets. These presets allow for storage of favorite speed and time settings for easy retrieval.
SHOPPING GUIDELINES: BioSpec Products was the first company to introduce bead mill cell disrupters to the scientific laboratory - some 30+ years ago. This method of cell disruption of unicellular organisms and small tissue samples has superceded most traditional cell disruption methods. In addition to BioSpec’s current five models of beadbeater cell disrupters, several other manufacturers now offer similar microvial-shaking, ‘beadbeater-type’ cell disrupters*. Most of of these shakers fulfill the following physical parameters that assure maximum cell disruption performance: A SHAKING SPEED of at least 2000 rpm; a THROW or displacement of the vial of 3/4 to 1 inch, a FIGURE "8" SHAKING PATTERN and a NEAR HORZONTAL ORIENTATION of the vial, tube or microplate. The full incorporation of these parameters maximizes bead circulation and bead collisions within the vial.
Side Note: Some, but not all, manufacturers are using shaking speed settings expressed as "meters/second". A machine setting in m/sec is not a comprehensive measurement of cell disruption efficiency. The term has caused buyer confusion in bead-mill product comparisons. And, in the scientific literature, makes it difficult to equate cell disruption protocols when using a different bead mill cell disrupter. There is no consensus performance term for shaking-type bead mill cell disrupters. The factors influencing cell disruption performance are many and their relationships to one another is complex.
BioSpec Products suggests that shaking cell disrupters machines be operated at their maximum available speed setting. Beadbeater applications requiring the option of lowerer operating speeds are rare. Put bluntly, if the objective is to disrupt cells, crank up the rpm of the machine and get the job done. The optimal time of beadbeating will depend on the nature of the biological sample and the type of bead mill shaker used. With current high performance machines, 1-3 minutes gives close to 100% cell disruption. If you are doing PCR work with nucleic acids and can settle for less than 100% cell disruption, shorter periods of beadbeating may suffice.
Besides the five variables shaking speed, vial throw distance, vial shaking pattern and orientation and time of shaking, three equally important variables which determine efficiency of cell disruption are bead size, bead composition and bead load in the vial. These later three variables must be optimized by the user, not only for the particular ‘beadbeater’ machine being used, but for the type of sample being investigated.
"Solutions" to deal with these last three variables are being offered in the form of commercially available vials prefilled with beads. There is little comparitive literature documenting how these vendors came up with their "magic mixtures".
One can usually get comparable results by loading your own beads into vials or microplates (and save a lot of money, too). Usually only one kind and size of bead will suffice. Our Bead Selection Guidelines give a straight forward presentation on how to select the correct bead size and composition and, if you have a lot of vials to load, BioSpec has three different bead loading devices designed to streamline the loading process.
* For contact information and a discussion of all commercially available bead mill cell disrupters see Cell Disrupters: A Review. In addition to shaking-type bead mill cell disrupters there are also vortexing-type bead mill cell disrupters. While the later function as intended, it can take 5 to 10X longer beadbeating times to get complete cell disruption. The exception is BioSpec Product’s new SoniBeast™ cell disrupter, which uses patented high speed vortex technology to disrupt cells up to 10X faster than ordinary shaking-type beadbeating machines.
Credits: The MiniBeadbeater-96 was introduced in 2001. It was designed by Horst Bachrowitz and was the first commercial beadbeater designed to disrupt cells using a microplate format. Time has proved it to be a high-throughput beadbeater of outstanding performance and reliability.