Description
BioSpec stainless steel microvials are engineered for demanding bead beating, dry grinding, cryogenic grinding, and high-energy sample disruption workflows where standard polypropylene microvials may crack or fail under extreme mechanical stress.
Manufactured from Type 305 stainless steel, these microvials maintain the same physical dimensions and approximate weight as standard 2 mL polypropylene microcentrifuge tubes, allowing compatibility with BioSpec Mini-Beadbeater systems and other bead mill homogenizers.
The microvials can be sealed using polyethylene flange caps, specially shaped silicone rubber stopper caps, or crimped 13 mm OD septa caps depending on the laboratory application and grinding conditions.
Common laboratory applications include:
- Dry grinding with steel beads in high-energy bead mill homogenizers where conventional plastic microvials may crack or rupture during processing.
- Cryogenic grinding and frozen sample disruption using dense steel bead media and liquid nitrogen temperature workflows.
- Improved PCR thermal cycling performance due to the superior thermal conductivity of stainless steel compared to plastic or glass sample vessels.
- Enhanced sample temperature control during bead beating, allowing faster cooling between homogenization cycles to minimize heat buildup in sensitive biological samples.
For many dry milling applications, BioSpec also offers reinforced 2 mL polypropylene XXTuff™ microvials, which are among the most durable polypropylene screw-cap microvials available for laboratory bead milling workflows.
Important: When bead beating dry or hard frozen samples using steel beads, BioSpec recommends using specially designed silicone rubber stopper caps to seal the stainless steel microvials. Standard polyethylene flange caps supplied with the vials may fail under severe bead milling conditions associated with dry grinding and cryogenic sample disruption.
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Other Info :
A protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. chrome-steel beads are added to a 2 ml stainless steel vial. The vial and beads are brought to liquid nitrogen temperature...without entrapping any liquid nitrogen inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is usually not necessary)*, quickly seal the vial with a non-frozen silicone rubber cap and grind for 5-15 seconds in a Mini-Beadbeater. The sample will be powdered.
If extraction of intracellular biomolecules is to follow, promptly add ice-cold liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for up to 3 minutes. The optimal beadbeating time will vary with sample type and should be empirically determined with an initial time study of 0.5, 1, 2, and 3 minutes of beadbeating.
* Add pre-frozen tissue. Fresh tissue will "stick" to the cold steel and delay good grinding.
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