Description

BioSpec XXTuff2™ reinforced microvials are designed to withstand the demanding conditions associated with dry grinding, bead beating, and high-impact tissue homogenization applications using dense grinding media such as stainless steel and chrome steel beads.

The XXTuff2 microvial maintains the same external dimensions as a standard 2 mL screw-cap microcentrifuge tube while incorporating reinforced structural regions in critical stress areas for significantly improved durability during laboratory homogenization workflows.

Testing demonstrates that the XXTuff2 vial is more than 10 times more durable than standard polypropylene microvials under demanding grinding conditions.

The updated XXTuff2 design improves upon earlier reinforced microvials by incorporating a skirted, free-standing bottom structure that helps reduce bottom blow-outs that may occur during certain dry grinding protocols. The vial also features a natural-colored screw cap for easy laboratory identification.

XXTuff2 reinforced microvials are autoclavable and certified DNase-free and RNase-free, making them suitable for molecular biology, genomics, RNA isolation, DNA extraction, and sensitive laboratory sample preparation applications.

Important: For CryoGrinding applications performed at liquid nitrogen temperatures, BioSpec stainless steel microvials are also recommended for maximum durability under extreme cryogenic grinding conditions.

  • Other Info :

    Protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. or two 6.25 mm dia. chrome-steel beads are added to either a 2 ml stainless steel vial or an XXTuff2 ml polypropylene vial.  The vial and beads are brought to liquid nitrogen temperature...without entrapping any liquid nitrogen inside the vial.  Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is not be necessary), quickly seal the vial and grind for 10-15 seconds in a Mini-Beadbeater.  The sample will be powdered.  Then promptly add liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for 0.5-3 minutes.  The optimal beadbeating time will vary with sample type and is empirically determined with a time study of 0.5, 1, 2, and 3 minutes of beadbeating.

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