Stainless Steel Microvials - 2 ml
Cat. No. 2007, 1.8 ml capacity, type 305 stainless steel microvials. Polyethylene flange caps are included1. Package size: 100 pieces
Description
Our Stainless Steel Microvials have the same physical dimensions and weight as standard 2 ml polypropylene microvials. They can be sealed with either polyethylene flange caps, silicone rubber stopper caps1 or crimped 13 mm OD septa caps.
Applications include:
- Dry grinding with steel beads in a high energy Bead mill cell disrupter: (e.g.,MiniBeadbeater) where milling forces can crack common plastic microvials - - an exception is our 2 ml polypropylene microvials. They are the toughest 2 ml screw-top polypropylene vial on the market and can be used for most dry milling applications.
- Improved PCR methods: Compared to plastic or glass vessels, stainless steel microvials provide superior temperature cycling capabilities.
- Control of sample heating during the beadbeating process: Stainless steel microvials support faster sample cooling steps that are sometimes required to offset the sample heating that occurs during beadbeating.
1 NOTE: When beadbeating dry or hard frozen samples using steel beads specially shaped Silicone Rubber Stoppers should be used to seal the stainless steel microvials. The flange caps, which come with s.s.microvials, can fail under these harsh bead milling conditions. Scroll down for more information on Silicone Rubber Caps.
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Other Info :
A protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. chrome-steel beads are added to a 2 ml stainless steel vial. The vial and beads are brought to liquid nitrogen temperature...without entrapping any liquid nitrogen inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is usually not necessary)*, quickly seal the vial with a non-frozen silicone rubber cap and grind for 5-15 seconds in a MiniBeadbeater. The sample will be powdered.
If extraction of intracellular biomolecules is to follow, promptly add ice-cold liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for up to 3 minutes. The optimal beadbeating time will vary with sample type and should be empirically determined with an initial time study of 0.5, 1, 2, and 3 minutes of beadbeating.
* Add pre-frozen tissue. Fresh tissue will "stick" to the cold steel beads and delay good grinding.
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Silicone Rubber Caps