SoniBeast - Small Sample Cell Disruptor
Extremely Fast Cell Lysis...<20 sec
- Perfect for Small Samples
- 12 Vial Capacity
- Simple One Button Operation
The NEW SoniBeast™ high energy bead-mill cell disruptor completely disrupts small samples of tissue or cells in seconds rather than minutes. It processes up to twelve 0.6 ml PCR microtubes, each containing 1-100 mg of sample, or three 2 ml microvials each containing 10-400 mg sample. The SoniBeast is designed for wet milling in water-based media. Common applications are the isolation of intracellular protein and nucleic acids.
A pack of polypropulene 0.6 ml locking cap PCR tubes is included with the SoniBeast. Additional Vials should be ordered separately. Beads size and composition vary with the kind of sample being disrupted. For guidance on choosing beads see Lysing Beads or give us a call at 918-336-3363.
Our Price : $0.00
The SoniBeast™* cell disrupter homogenizes both microorganisms and plant and animal tissue using the smashing action of colliding ceramic beads (commonly called beadbeating). It departs from contemporary beadbeater machines by oscillating microtubes at a rate of 20,000 oscl/min...some 5 to 50 times faster than currently available beadbeating machines.
As expected, cell disruption occurs much faster when beads inside the tubes are moving at extraordinarily high rates. Disruption is completed in a few seconds rather than minutes. Faster disruption times favor higher quality and yields of nucleic acids and proteins because all cells in a tissue sample reach the protection of the extraction media much quicker. The operation of SoniBeast cell disrupter has also been simplified compared to conventional beadbeaters. The machine is operated by the push of a single button. No required manual clamping of tubes or vials onto the machine is required and the physical location of vials remain unchanged from the starting point. Thus, the SoniBeast is the first high-efficiency bead-mill cell disrupter offering compatibility with robotic systems.
The SoniBeast's tray capacity is one to twelve 0.6 ml PCR microtubes, each containing up to 100 mg of sample. Closer to the axis of the machine is an inner ring having six positiions that holds larger 1.5 or 2 ml microvials capable of processing up to 400 mg of sample. Samples in these larger microvials are lysed under the milder grinding conditions that emulate earlier published beadbeating protocols.
- Processes one to twelve 0.6 ml PCR tubes each holding 1-100 mg (wet weight) of tissue or cells at ultra-high shaking energy or one to three 2 ml screw-cap microvials each holding 10-400 mg (wet weight) tissue or cells at conventional beadbeater shaking energy.
- Motor Specifications: 110-120 V~AC, 1.6A, 21,000 rpm
- Manual pulse or timed operation (0.5, 2, 5, 10, 20, 40, and 60 sec with auto-reset). Activate with the push of a single button.
- Footprint: 5" wide x 16" deep. Seven inches high. Weight 11 pounds
- Two year Total Warranty. Eventual motor replacement is anticipated with this very high energy machine - especially with high usage. The SS is disigned to facilitates a fast, easy, on-site change out.
General Protocol. One to twelve 0.6 ml microtubes are sequentially loaded with beads, tissue sample and extraction media. After capping, they are inserted into the red silicone rubber grommets in the disc-shaped tube holder. Unlike all other beadbeaters, inserted tubes do not need to be clamped down.. After selecting a run time (typically 5-20 seconds) the SoniBeast is activated with the touch of a single button. Seconds later the SoniBeast turns off, the beads fall by gravity to the bottom of the vial, and the lysed tissue sample(s) is transfered by micropipette for subsequent purification and/or analysis.
See https://biospec.com/instructions/sonibeast for detailed operating instructions.
Quicker, Simpler, Less Consumables, Smaller Footprint, Affordable.