Screw Tissue Press
The Screw Tissue Press is a classic screw press, designed to efficiently macerate or mechanically disaggregate 5 to 50 grams of fresh, soft plant or animal tissue.
Tissue processed in this press must be "soft", relatively non-fibrous and have high water content. Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers. Tough fibrous tissues like leaves, skin or bone will not pass through the stainless steel screen.
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The Screw Tissue Press prepares tissue as an initial mincing step preceeding mechanical homogenization (cell disruption) or preceeding single cells isolation from solid tissue by digestion of extracellular connective matrix with proteolytic enzyme. The press eliminates tedious chopping of tissue into little pieces with a scissors or scalpel and produces much smaller tissue pieces. Unlike many other tissue dispersion/blending devices, no buffer is added in its operation.
Disaggregation of tissue for primary culture benefits: Incubation time needed for enzyme assisted tissue disaggregation is reduced and, because the much smaller size of tissue pieces, diffusion of nutrients and gases into the tissue mass is optimized during the incubation period with the digestive enzymes. This results in higher yields of viable cells. [See http://www.worthington-biochem.com/tissuedissociation/default.html) for an excellent review of the Method of Enzymatic Disaggregation].
1) Acceptible tissue is soft, relatively non-fibrous and has high water content. Examples are muscle, brain, liver, plant fruits, some plant roots and tubers. If the tissue is partially fibrous, fatty or covered with a tough membrane, first manually trim off the tough or fatty parts. Fibrous tissue will interfere with the passage of the soft tissue through the press and lower the yield of dispersed cell material. Skin, hide, connective tissue, plant stems, wood and dried tissues cannot be processed.
2) Partially retract the piston cylinder of the Screw Tissue Press to make room for the tissue. Unscrew the brown 'cap' with its array of 2 mm holes from the bottom of the Screw Tissue Press and place the tissue sample into the cavity of the press.
Generally, the array of 2 mm diameter holes in the press cap is used. If your sample is soft, non-fibrous material with high water content, you can also use the 500 micron stainless steel wire sieve (included). Carefully center it on the end of the press cylinder after adding the sample tissue ans screw on the press cap.
3) Manually turn the piston to extrude the tissue. The dispersed tissue will exit the press having a paste- or granular-like texture. Depending on the amount of connective or fibrous tissue present in the sample, the yield of dispersed tissue will vary from 20-95%.
4) Extruded tissue material can be easily removed from the end of the Screw Tissue Press with a scalpel or single edge razor blade.