Our 2.0 ml, screw cap with o-ring seal, microcentrifuge vials are made of inert polypropylene and have straight walls with a sharp conical bottom. This vial shape is required for the Mini-Beadbeater-1 and recommended for the MiniBeadbeater-16, MiniBeadbeater-24 and MiniBeadbeater-96. The vials are available sterile or non-sterile.
For extraction and cell disruption of larger sample volumes we also offer 7 ml polypropylene vials. These vials include screw caps and, when combined with the appropriate adapter, can be used with the Mini-Beadbeater-16, -24, or -96.
Note: Using our high-energy Minibeadbeater series cell disrupters, current polypropylene microvials (offered by us or anyone else) cannot be used to steel beads. The steel beads will crack or punch through the vials. No problem exists using zirconia or glass beads for dry milling or steel beads for <span underline;"="">wet milling. If dry milling with steel beads is desired (commonly done at liquid nitrogen temperatures and called cryo-grinding - see example protocol below), use our stainless steel microvials and silicone rubber stoppers or a much tougher polypropylene 'XXTuff™' 2 ml microvial. The later is reinforced in critical vial areas and molded with a special, high density polypropylene resin. Tests show that it is 10X more resistant to breakage - hence the name 'XXTuff'.
Protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. or two 6.25 mm dia. chrome-steel beads are added to either a 2 ml stainless steel vial or an 'XXTuff' 2 ml polypropylene vial. The vial and beads are brought to liq N2 temperature...without entrapping any liq N2 inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is not be necessary), quickly seal the vial and grind for 10-15 seconds in a MiniBeadbeater. The sample will be powdered. Then promptly add liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for 0.5-3 minutes. The optimal beadbeating time will vary with sample type and is empirically determined with a time study of 0.5, 1, 2, and 3 minutes of beadbeating.