Lever Tissue Press
The Lever Tissue Press is a classic garlic press which has been enhanced by addition of a silicone-rubber gasket the lever press piston and the inclusion of an extra "can" to facilitate processing of multiple samples. It efficiently macerates or mechanically disaggregates 5 to 50 grams of fresh, soft plant or animal tissue. By 'soft' we mean nonfibrous tissue. Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers. Tough or fibrous tissues like leaves, skin or bone will not pass through the holes in the stainless steel can..
Our Price : $0.00
The Lever Tissue Press prepares tissue as an initial mincing step preceeding mechanical homogenization (cell disruption) or preceeding single cells isolation from solid tissue by digestion of extracellular connective matrix with proteolytic enzyme. The press eliminates tedious chopping of tissue into little pieces with a scissors or scalpel and produces much smaller tissue pieces. Unlike many other tissue dispersion/blending devices, no buffer is added in its operation.
Disaggregation of tissue for primary culture - the benefits: Incubation time needed for enzyme-assisted tissue disaggregation is reduced and diffusion of nutrients and gases into the tissue mass during the incubation period with the digestive enzymes is optimized. Expect higher yields of viable cells. For an excellent review of the Method of Enzymatic Disaggregation see http://www.worthington-biochem.com/tissuedissociation/default.html) .
Tissue processed in this press must be "soft", relatively non-fibrous and have high water content. Acceptible examples are muscle, brain, liver, plant fruits, and some plant roots and tubers. If the tissue is fatty or partially fibrous, manually trim off the tough or fatty parts before pressing. Fibrous tissue will interfere with the passage of the soft tissue through the press and lower the yield of dispersed cell material. Unacceptible tissues are skin, hide, connective tissue, woody or fibrous plant stems, many mature seeds and all dried tissues.
1) Retract the piston of the Lever Tissue Press from its stainless steel "can". Add 1 to 10 g of fresh tissue into the cavity of the "can" and swing the piston back into position.
2) Grip the two levers and force the piston down the can. Scrape the dispersed tissue protruding from the bottom of the can with a single edge razor blade or scapel. Depending on the amount of connective or fibrous tissue present in the sample, the yield of dispersed tissue will vary from 20-95%.