Lever Tissue Press

The Lever Tissue Press is used as a mincing step for isolation of single cells or the extraction of intracellular components such as nuclei and mitochondria. Unlike other tissue dispersion/blending devices, no added water or buffer is required in its operation.  See Narendra P Singh, et. al. for preparation of viable single cell suspensions  [DNA double-stranded breaks in mouse kidney cells with age, Narendra P Singh et. al., Biogerontology 2, 261-270 (2001)].

The Lever Tissue Press, a modified garlic press, enhanced by addition of a silicone-rubber gasket to the bottom of the lever press piston and the inclusion of an extra "can" to facilitate processing of multiple samples.

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The Lever Tissue Press is used as a mincing step for isolation of single cells or the extraction of intracellular components such as nuclei and mitochondria. Unlike many other tissue dispersion/blending devices, no water or buffer is added in its operation.

Disaggregation of tissue for primary culture:  A common method to isolate single cells from solid tissue samples involves digestion of extracellular connective matrix with proteolytic enzymes.  The first step in most protocols is to manually chop the tissue into little pieces with a scissors or scalpel in order to increase access of the enzymes to the tissue surface.  The Lever Tissue Press eliminates this tedious manual chopping and delivers a significant improvement by producing much smaller tissue pieces.  [For an example of preparation of viable single cell suspensions see DNA Double-stranded Breaks in Mouse Kidney Cells with Age, Narendra P Singh et. al., Biogerontology 2, 261-270 (2001)].

Benefits: Incubation time needed for enzyme assisted tissue disaggregation is reduced and, because the much smaller size of tissue pieces, diffusion of nutrients and gasses into the tissue mass is optimized during the incubation period with digestive enzyme.  This results in higher yields of viable cells.  [See http://www.worthington-biochem.com/tissuedissociation/default.html) for an excellent review of the Method of Enzymatic Disaggregation].

Operating Instructions:

Retract the piston of the Lever Tissue Press from its stainless steel can.  Add 1 to 10 g of fresh tissue to the cavity of the stainless steel can and swing the piston into position.  Grip the levers together, thus forcing the piston down the press can or barrel.  The dispersed tissue protruding from the bottom of the can is removed with the swipe of a single edge razor blade or scapel.

Note: Tissue must be "soft", relatively non-fibrous and have high water content.  Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers.  If the tissue is fatty or partially fibrous manually trim off the tough or fatty parts before pressing.  Fibrous tissue will interfere with the passage of the soft tissue through the press and lower the yield of dispersed cell material.  Unacceptible tissues are skin, hide, connective tissue, woody or fibrous plant stems, many mature seeds and all dried tissues.