Lever Tissue Press
The Lever Tissue Press is a modified garlic press. Dozens of domestic garlic presses were tested in our laboratory for dispersive efficiency, ease of cleaning and ruggedness of construction. The top performing press was then further enhanced by addition of a silicone rubber gasket to the bottom of the lever press piston. An extra "can" is also included to facilitate processing multiple samples.
Our Price : $0.00
The Lever Tissue Press is used as a mincing step for isolation of single cells or the extraction of intracellular components such as nuclei and mitochondria. Unlike other tissue dispersion/blending devices, no added water or buffer is required in its operation. See Narendra P Singh, et. al. for preparation of viable single cell suspensions [DNA double-stranded breaks in mouse kidney cells with age, Narendra P Singh et. al., Biogerontology 2, 261-270 (2001)].
Disaggregation of tissue for primary culture: A common method to isolate single cells from solid tissue samples involves digestion of extracellular connective matrix with proteolytic enzymes. The first step in most protocols is to manually chop the tissue into little pieces with a scissors or scalpel in order to increase access of the enzymes to the tissue surface. The Lever Tissue Press eliminates this tedious manual chopping and delivers a significant improvement by producing much smaller tissue pieces.
The incubation time needed for enzymatic tissue disaggregation is reduced and, because the size of tissue pieces are much smaller, diffusion of nutrients and gasses into the tissue mass is less limiting during the incubation period with digestive enzyme. This results in higher yields of viable cells. (see http://www.worthington-biochem.com/tissuedissociation/default.html) for an excellent review on the method of Enzymatic Disaggregation)
Retract the piston of the Lever Tissue Press from its stainless steel can. Add 1 to 10 g of fresh the tissue to the cavity of the stainless steel can and swing the piston into position. Grip the levers and force the piston down the press can or barrel. Dispersed material protruding from the bottom of the can is easily removed with the swipe of a single edge razor blade or scapel.
Note: Acceptible tissue is soft, relatively non-fibrous and has high water content. Examples are muscle, brain, liver, plant fruits, some plant roots and tubers. If the tissue is partially fibrous, fatty or covered with a tough membrane, first manually trim off the tough or fatty parts. Fibrous tissue will interfere with the passage of the soft tissue through the press and lower the yield of dispersed cell material. Skin, hide, connective tissue, plant stems, wood and dried tissues cannot be processed.