The CryogenicTissueGrinder is a high-speed blade mill that grinds 1-10 grams of microbial, plant, animal and human tissue having high water content (~70%) to a fine powder in the presence of excess dry-ice. At solid CO2 temperatures, the hard and brittle sample is powdered in seconds. Pulverizing tissue at very low temperature stabilizes intercellular biochemicals and prepares the tissue for rapid extraction in an appropriate liquid media.
Cat. No. CTG111, 120 volts
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Tissue samples are simultaniously frozen and fragmented in seconds with the Cryogenic Tissue Grinder. No heat is generated during grinding. Nucleic acids, proteins and other biochemicals in the cell are preserved unaltered by the grinding process. The powdered tissue can be separated from the dry-ice "snow" by lyophilization under vacuum or simply by storing the mixture in the freezer compartmet of a refrigerator while the dry-ice sublimes away overnight. Powdering tissue shortens the time of extraction with chemical reagents or mechanical devices. It also enhances penetration of tissue with 'RNAlater' or other like expedients.
This method works best with highly hydrated tissue. Tissues and materials such as wood, hair, feathers, hide, bone and some seeds may not cryogrind well with the CTG111. With these tougher tissues consider using devices which exploit a related process of cryopulverization.
Operating the CTG111
Pieces of dry-ice (available from local grocery stores) equal to 1 to 5 times the volume of the tissue is placed in the stainless steel cup of the CTG grinder. Pre-powder the dry ice in the CTG111 for a couple of seconds before adding tissue. With the tissue added, the grinder is operated at maximum speed for 5-10 seconds. Pulse operation and tapping the sides of the grinding chamber during operation is helpful.
Using a modified application for microorganisms, a thick, "paste" suspension of yeast or bacteria is extruded from a syringe onto pre-powdered dry-ice where it quickly forms frozen "spaghetti'. Alternatively, hard-frozen yeast "pellets" are prepared by dripping the microbial "paste" into a container containing liquid nitrogen. After most of the liq N2 has evaporated, the still frozen yeast pellets are transfered to the Cryogenic Tissue Grinder containing dry-ice for powdering and cell lysis. The time need for complete cryopulveriation should be determined empirically by doing a time study.
Finally, the mixture of dry-ice and cryopulverized tissue or microorganism is stored in the freezer compartment of a refrigeraor where the dry-ice is allowed to sublime overnight. What remains a powder of lysed cells.
- Removable grinding chamber is stainless steel and dishwasher and autoclave safe
- Hands-free operation
- Built-in adjustable timer
- Compact size, L: 5 in, H: 9 in, W: 5 in
- 120 volts, 60 Hz, 210 watts