The CryogenicTissueGrinder is a high speed blade mill that grinds 0.5-10 grams of plant, animal and human tissue with high water content to a fine powder in the presence of dry ice. The tissue becomes brittle at solid CO2 temperatures. A sample hit with a hammer (or in the case of the CTG111, a spinning blade) fragments tissue in seconds.
Cat. No. CTG111, 120 volts
Our Price : $0.00
Tissue samples are simultaniously frozen and fragmented in seconds with the Cryogenic Tissue Grinder. No heat is generated during grinding. Nucleic acids, proteins and other biochemicals in the cell are preserved unaltered by the grinding process. The powdered tissue can be separated from the dry-ice "snow" by lyophilization under vacuum or simply by storing the mixture in the freezer compartmet of a refrigerator while the dry-ice sublimes away overnight. Powdering tissue shortens the time of extraction with chemical reagents or mechanical devices. It also enhances penetration of tissue with 'RNAlater' or other like expedients.
This method works best with highly hydrated tissue. Tissues and materials such as wood, hair, feathers, hide, bone and some seeds may not cryogrind well with the CTG111. With these tougher tissues consider using devices which exploit a related process of cryopulverization.
Operating the CTG111
Pieces of dry ice (available from some local grocery stores) equal to 1 to 5 times the volume of the tissue is placed in the stainless steel cup of the grinder. Pre-powdering the dry ice in the CTG111 for a second before adding tissue is recommended, especially with fresh, soft, "wet" tissue. With the tissue added, the grinder is operated at maximum speed for 5-10 seconds. Tapping the sides of the grinding chamber during operation is helpful.
In a special application for microorganisms, a thick, "paste" suspension of yeast or bacteria is extruded from a syringe onto pre-powdered dry ice where it forms frozen "spaghetti'. The time will be longer and optimal cell disruption time should be determined empirically by doing a time study.
Using a similar technique, hard-frozen yeast "pellets" are prepared by dripping the microbial "paste" into a container containing liquid nitrogen. After most of the liq N2 has evaporated, the frozen yeast pellets are transfered to the Cryogenic TissueGrinder for final powdering and cell lysis.
- Removable grinding chamber is stainless steel and dishwasher and autoclave safe
- Hands-free operation
- Built-in adjustable timer
- Compact size, L: 5 in, H: 9 in, W: 5 in
- 120 volts, 60 Hz, 210 watts