An important accessory for the MiniBeadbeater-96 when temperature control is a concern**. Typically, a vial filled with beads, sample and extraction media warms up ~10 degrees per minute of beadbeating. Our 2 ml vial holder (capacity 48 vials) is machined out of high-heat-conductivity aluminum. Before use in the Mini-BeadBeater-96, the vial holder is pre-cooled to low temperatures inside a refrigerator, a -80º C freezer, or with liquid nitrogen. During the beadbeating process the cold vial holder keeps the sample temperature at a desired low temperature. By comparison, the cooling efficiency of a well chilled, close-fitting, solid aluminum vial holder is far superior to air in a cold-room or cold air derived from an air-cooling attachment offered from some bead mill cell disruptor manufacturers.
In a related application, 'dry' bead-grinding of fresh tissue by the cryopulverization technique is possible. The sample, beads, vials, and vial holder are all pre-chilled to liquid nitrogen temperatures before being processed in a Mini-BeadBeater-96.
** Helpful Note: Nucleic acid extraction of microorganisms or tissues in the presence of nucleic acid extraction media generally does not require temperature control.