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Pulverizers

Pre-Chopping. No matter what mechanical device is used to homogenize or lyse tissue (bead-mill, tissue grinder, rotor-stator device, ultrasonicator, high-pressure press, etc) the tissue will process faster if it is first reduced to very small pieces (<1 mm pieces).

If the tissue is fresh, size reduction can be done by repetitive chopping with a scalpel or single-edge razor blade.  Another way to pulverize tissue is to freeze it in liq N2 and impact it with with a hammer or a spinning blade.  Called cryopulverization, the method is fast and improves the yield of proteins or nucleic acids after subsequent homogenization steps.

Tools to cryopulverize tissue...

BioCryo Tissue Grinder

BioCryo Tissue Grinder

The Bio Cryo Tissue Grinder is a high-speed blade mill. The tightly sealed, 3/4 cup capacity (~200 ml) grinding chamber has a sealed pour spout for optional addition and removal of extraction liquids after "dry" grinding.

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BioPulverizer

BioPulverizer

The BioPulverizer quickly fragments hard frozen tissue samples into tiny pieces the size of grains of sand or course powder.  The primary benefit of pulverizing pieces of tissue is to optimize subsequent rapid and complete cell lysis using lytic solutions or mechanical homogenizers. For difficult tissues such as tendon, skin, and tumors it is an essential step (read more). The BioPulverizer is also used at room temperature to fragment friable, dry biomaterial such as non-oily seeds, bone and teeth.
  
There are four different hand-operated BioPulverizers for quickly pulverizing 10 mg to 10 g of frozen tissue.

Cat. No. 59012N, BioPulverizer, capacity 10-100mg
Cat. No. 59013N, BioPulverizer, capacity 0.1-1g
Cat. No. 59014N, BioPulverizer, capacity 1-10g
Cat. No. 59012MS, MultiSample BioPulverizer, 12 wells, capacity 10-100mg per well*

*Twelve pestles are included with each 59012MS.

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BioSqueezer Snap-Freeze Press

BioSqueezer Snap-Freeze Press

The BioSqueezer "snap-freezes" 10-100 mg of soft animal and human tissue.  Extremely rapid freezing of the sample produces a glassy state called vitrification - without water crystalization.  Applications include the study of rapid events in cell metabolism, improved tissue cryopreservation and recovery from cryobanking, and preparation for cryo-pulverization and cryo-grinding.  The BioSqueeezer is easy to use and provides reproducible results.

Fresh tissue is compressed into a thin disc about the size of a small coin under pressure from a stainless steel clamp head that has been pre-cooled in liquid nitrogen or dry ice.   Freezing occurs in a few milliseconds, thus offering a "snapshot" of metabolic events in the cells.  The hard frozen disc is easily fragmented by grinding and subsequent rapid extraction of labile nucleic acids**, proteins, and other intracellular products.

** Very high molecular weight DNA is obtained by snap-freezing and then cryo-pulverizing the hard-frozen tissue using one of our milling devices (Cryo-Cup mortar and pestle, BioPulverizer, or MiniBeadbeater) followed by passive extraction (i.e., minimized physical stirring, pipetting, or agitation during the cell lysis in DNA extraction media).

 

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Cryo-Cup Grinder

Cryo-Cup Grinder

The Cryo-Cup™ Grinder is a mortar and pestle specifically designed to pulverize and powder plant and animal tissue samples at liquid nitrogen temperatures.  It gives superior results compared to conventional ceramic mortar-pestles.

Cat. No. 206, Cryo-cup™ Grinder, complete. Includes two different size polypropylene pestle balls. See below for extra cups (mortars) and pestle balls.

Cat. No. 206C, Extra s.s. mortar with insulated cup. (Pestle not included)
Cat. No. 206PP138, Polypropylene pestle ball, 1  3/8 inch dia.
Cat. No. 206PP178, Polypropylene pestle ball, 1  7/8 inch dia.
Cat. No. 206SS138, Stainless steel pestle ball, 1  3/8 inch dia.
Cat. No. 206SS178, Stainless steel pestle ball, 1  7/8 inch dia.
Cat. No. 206H, Spare handle for use with pestle ball

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MicroCryoCrusher

MicroCryoCrusher

Hand-operated device especially useful for crushing bone and tooth samples.

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Mini Leaf Crusher

Mini Leaf Crusher

The Mini Leaf Crusher™ is a hand-held clamp designed for sampling plants and their pathogens in the field and other locations distant from the analytical laboratory.  Used in combination with its unique BioSpec DNA Sample Card™, the intracellular contents of leaves, stems, and root tissue are crushed by clamping at thousands of pounds per inch²,  The released cell extract is immediately captured and stabilized on special cellulose filter paper located within the compact Sample Card.  Multiple samples can be collected in a day and no preservatives or low-temperature storage is required for transit of the card(s) to the laboratory for subsequent RNA or DNA analysis.

Using published techniques, the bound nucleic acids can be then be extracted in minutes from the special filter paper in the Card in a purified state ready for direct application in downstream PCR-based methodologies or genome sequencing.  The Card alone has additional applications.  It is used to stabilize and transport microliter quantities of blood, sputum, urine, and homogenized processed foods.

The Mini Leaf Crusher comes with an introductory five-pack of BioSpec DNA Sample Cards.  Additional Sample Cards can be ordered separately.

Cat. No. MLC320, Mini Leaf Crusher includes 5 BioSpec DNA Sample Cards

Cat. No. DSC20, BioSpec DNA Sample Cards, 20 pack

Cat. No. DSC50, BioSpec DNA Sample Cards, 50 pack

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