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Medium-scale Tissue Extraction Tools - A Selection Table

1 Liter Hamilton Beach Blender

1 Liter Hamilton Beach Blender

Hamilton Beach is the worlds largest manufacturer of commercial grade blenders. The model 909 comes with a one liter stainless steel container and can handle the toughest job required from a blade blender.

Cat. No. 909, 115 volt model
Cat. No. 909EUR, 230 volt model

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BeadBeater

BeadBeater

The BeadBeater is the ultimate laboratory-scale homogenizer for disrupting aqueous suspensions of yeast, fungi or bacteria.  Complete cell lysis of 10-80 g (wet weight) of cells is achieved in about three minutes of operation.  The BeadBeater bead-mill methodology is considered superior to other mechanical methods of microbial cell disruption.   And, in most cases, it is the most economical.

The BeadBeater comes complete with a 350 ml polycarbonate chamber, rotor assembly, motor base, ice-water cooling jacket and one pound of glass beads.

If the BeadBeater is to be used in Europe or parts of Asia where the power supply is 220-240 VAC, please select the BeadBeater catalog number containing " EUR ".

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BioHomogenizer

BioHomogenizer

The BioHomogenizer is a rotor-stator type tissue homogenizer designed to rapidly homogenize, disperse, and emulsify biological samples in aqueous media from 1 to 1000 ml.  The BioHomogenizer includes both the motor and probe.

Cat. No. 1281, BioHomogenizer for 1-200ml samples, complete with 1.4 cm probe
Cat. No. 1281EUR, 230 volt version of No. 1281
Cat. No. 1285, BioHomogenizer for 100-2000ml samples, complete with 2.5 cm probe
Cat. No. 1285EUR, 230 volt version of No. 1285

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Electric Juicer

Electric Juicer

Ideal for a one step preparation of cell lysate from most fruits and vegetables.  Suitable tissues include tubers, squash, leafy tissues, fruits and pods.  One of the few methods that do not require added liquid to obtain a cell free extract.  Consequently, the highly concentrated proteins and biochemicals in the homogenate optimize down-stream purification or analysis steps.  Not suitable for extracting animal tissue.  

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Electric Meat Grinder

Electric Meat Grinder

The meat grinder reduces fresh muscle and animal organ tissue to piece about 1 mm in cross section. The cutting action is much like that of a pair of scissors.  During this gentle dispersion process a high proportion of the tissue cells remain intact and viable. This classic tool has two applications in the molecular biology area:  1) Preparing large amounts tissue (100- >1 kg) for subsequent fast and complete cell lysis using a beadbeater, ultrasonicator, rotor-stator machine or high shear press and 2) recovering large quanties of viable single cells following incubation of the dispersed tissue with proteolytic enzymes that digest extracellular matrix.

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Seed Flour Mill

Seed Flour Mill

The electric flour mill is a high speed impact-type mill.  Up to 2 liters of dry, non-oily seeds are quickly reduced to a fine flour-like powder.  Subsequent solvent extraction of the high-surface area powder gently releases enzymes, proteins and other intracellular biochemicals. Examples of suitable seeds and grains are wheat, rye, rice, millet, corn, barley, dried beans and peas.  Importantly, moisture content and oil content of the seeds should not exceed 15%. Examples of unsuitable seeds are sunflower, nuts, sesame, coffee, and peanuts.

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Semi-Continuous Tissue Disperser

Semi-Continuous Tissue Disperser

A top-of-the-line domestic waste disposer modified to sit on a lab bench.  Close loop plumbing has been added to recycle the contents.  The Semi-Continous Tissue Disperser delivers a high quality homogenate of almost any plant or animal material.  Kilogram quantities of material can be processed in minutes using a batch-wise or in a semi-continuous mode.  Can be operated at room temperature or a cold room.

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