Bead Mill (Beadbeater) Cell Disrupters
Bead Milling (Beadbeating) is the preferred general laboratory method for disruption of tissue cells and microorganisms for the recovery and analysis of intracellular proteins, nucleic acids and organelles. It's fast, efficient, and very bio-safe. Accessories to control sample heating during cell disruption are sometimes advised.
First to introduce to the R&D laboratory in 1979, BioSpec Products produces five models of beadbeater. Each is optimized for different sample size and vial capacity. Depending on the model chosen, 20 to 2000 samples can easily be processed in an afternoon. The models are subdivided into three catagories based on the way the beads are agitated: End results obtained from each Beadbeater model are very similiar.
The NEW SoniBeast™ bead mill uses a patent-pending bead mixing technology. It delivers much faster cell disruption (up to 10X) compared to conventional bead mill-type cell disrupters on the market (including our MiniBeadbeaters) and is designed to process smaller samples. The SoniBeast's capacity is twelve 0.6 ml microtubes or three 2 ml microvials.
There are four models of the Mini-Beadbeater™ cell disrupter. All use standard 3/4" - 1", back-and-forth (top to bottom) shaking, wirh a "figure-8" pattern and have ben engineered to offer protcol-compatibity within the group. The new MBB1-Plus processes one 2 ml microvial at a time. The MBB-16 and MBB-24 cell disrupters have multi-vial capacities and can use both 2- and 7-ml microvials. The high through-put MBB-96 processes either one or two deep well microplates, a rack containing up to 45 microvials, or a rack holding two 50 ml screw-cap tubes. Acceptible sample sizes for this group of disruptors range from 1 mg to 1.5 g (wet weight) of tissue or packed cells. Mini-Beadbeater shaking energies are equal or exceed all competitive brands of shaking-type bead mill.
The BeadBeater™ is a larger capacity beadbeater that can processes 10-80 g (wet weight) of microorganisms or monocellular plant or animal cells. Rather than shaking motion, a fast turning Teflon rotor agitates the beads within a sealed chamber. It is commonly used to disrupt cell suspensions such as yeast or bacteria for production of native or expressed intracellular proteins. Unlike most other cell disruption methods used in the laboratory, the "Beadbeater method" is easily scaled up to pilot plant and full production levels.
Product descriptions follow...
The BeadBeater is the ultimate laboratory-scale homogenizer for disrupting aqueous suspensions of yeast, fungi or bacteria. Complete cell lysis of 10-80 g (wet weight) of cells is achieved in about three minutes of operation.
The BeadBeater comes complete with a 350 ml polycarbonate chamber, rotor assembly, motor base, ice-water cooling jacket, and your choice of one pound of glass beads...
Select the catalog number ending in...
-101 for 0.1 mm glass beads
-105 for 0.5 mm glass beads
-110 for 1.0 mm glass beads
Also, if the BeadBeater is to be used in countries with a 220-240 volt power supply, please select the catalog number containing "EUR."
The Mini-Beadbeater-24 disrupts up to 24 microbial or tissue samples with better than 95 percent efficiency. Cells are disrupted quickly and safely in the sealed system. The apparatus is easy to clean, has a small footprint and is essentially maintenance free. Compared to the Minibeadbeater-16, the Mini-24 adds advanced electronics for enhanced motor function, variable speeds of 2400-3800 rpm and a 30% increase in microvial capacity.
Cat. No. 112011, Mini-BeadBeater-24, 115 volt
Cat. No. 112011EUR, Mini-Beadbeater-24, 230 volt
European buyers! The Mini-Beadbeater-24 is CE certified.
The Mini-Beadbeater-96 is high-energy, high-throughput cell disrupter. It can process one 2 ml deep well micro plate (96 samples), two 1 ml deep well microplates (192 samples) or, using a included 45 capacity polypropylene microvial rack, 0.6, 1.5 or 2 ml microvials. In a busy day thousands of samples can be processed. Accessory 2 ml or 50 ml vial holders machined out of a solid aluminium blocks are availablle for strict temperature control, both at ice and liq N2 temperatures. See 'Features' tab, below, for more.
Cat. No. 1001, Mini-Beadbeater-96, 115 volt.
Cat. No. 1001EUR, Mini-Beadbeater-96, 230 volt.
Beads and vials vary with the application and should be ordered separately. For selecting Beads and Vials see their links in Browse box in the left margin.
The Mini-Beadbeater-Plus™ is an upgraded version of the venerable Mini-BeadBeater-1. Improvements include a programmable timer, a powerful brushless motor and 115/230 VAC compatibility. The high-energy bead-mill rapidly and completely disrupts microorganisms and plant and animal tissue.
Cat. No. 1018, Mini-BeadBeater-Plus, 110/220 volt
Note: Vials and beads required for the MBB Plus will vary with the particular sample being disrupted and should be purchased separately. For guidance on selecting Beads and Vials see links in Browse box in the left margin of this web page. If you would like to discuss your application with a technical expert please e-mail us or give us a call at 918-336-3363.
Extremely Fast Cell Lysis...<20 sec
- Perfect for Small Samples
- 12 Vial Capacity
- Simple One Button Operation
The NEW SoniBeast™ high energy bead-mill cell disruptor completely disrupts small samples of tissue or cells in seconds rather than minutes. It processes up to twelve 0.6 ml PCR microtubes, each containing 1-100 mg of sample, or three 2 ml microvials each containing 10-400 mg sample. The SoniBeast is designed for wet milling in water-based media. Common applications are the isolation of intracellular protein and nucleic acids.
A pack of polypropulene 0.6 ml locking cap PCR tubes is included with the SoniBeast. Additional Vials should be ordered separately. Beads size and composition vary with the kind of sample being disrupted. For guidance on choosing beads see Lysing Beads or give us a call at 918-336-3363.