Bead Mill Cell Disrupters
Bead Milling (Beadbeating) is the preferred laboratory method for disruption of cells to recover intracellular proteins, nucleic acids and organelles. It's fast, efficient, and safe.
First to introduce this method of cell disruption to the R&D laboratory in 1979, BioSpec Products now produces five different models of beadbeaters. Each is optimized for different sample size and capacity. Depending on the model chosen, 20 to 2000 samples can easily be processed in an afternoon. The models are divided into three catagories based on the way the beads are agitated:
The NEW SoniBeast™ bead mill uses a patent-pending bead mixing technology. It delivers much faster cell disruption (up to 10X) compared to conventional bead mill-type cell disrupters and can process smaller samples. The SoniBeast's capacity is twelve 0.6 ml microtubes or six 2 ml microvials.
There are four models of the Mini-Beadbeater™ cell disrupter. All use standard 3/4" - 1", back-and-forth shaking, wirh a "figure-8" pattern and have ben engineered to offer protcol-compatibity within the group. The new MBB1-Plus processes one 2 ml microvial at a time. The MBB-16 and MBB-24 cell disrupters have multi-vial capacities and can use both 2- and 7-ml microvials. The high through-put MBB-96 processes either one or two deep well microplates, a rack containing up to 45 microvials, or a rack holding two 50 ml screw-cap tubes. Acceptible sample sizes for this group of disruptors range from 1 mg to 1.5 g (wet weight) of tissue or packed cells. Mini-Beadbeater shaking energies are very high and equal to or exceed all competitive brands of shaking-type bead mill.
The BeadBeater™ is a larger capacity beadbeater that can processes 10-80 g (wet weight) of microorganisms or monocellular plant or animal cells. Rather than shaking motion, a fast turning Teflon rotor agitates the beads within a sealed chamber. It is commonly used to disrupt cell suspensions such as yeast or bacteria for production of native or expressed intracellular proteins. Unlike most other lab cell disruption methods used, the "Beadbeater method" can easily be scaled up to pilot plant and full production levels.
Product descriptions follow...
The BeadBeater is the ultimate laboratory-scale homogenizer for disrupting aqueous suspensions of yeast, fungi or bacteria. Complete cell lysis of 10-80 g (wet weight) of cells is achieved in about three minutes of operation.
The BeadBeater comes complete with a 350 ml polycarbonate chamber, rotor assembly, motor base, ice-water cooling jacket, and your choice of one pound of glass beads...
Select the catalog number ending in...
-101 for 0.1 mm glass beads
-105 for 0.5 mm glass beads
-110 for 1.0 mm glass beads
Also, if the BeadBeater is to be used in countries with a 220-240 volt power supply, please select the catalog number containing "EUR."
The Mini-Beadbeater-24 disrupts up to 24 microbial or tissue samples with better than 95 percent efficiency. Cells are disrupted quickly and safely in the sealed system. The apparatus is easy to clean, has a small footprint and is essentially maintenance free. Compared to the Minibeadbeater-16, the Mini-24 adds advanced electronics for enhanced motor function, variable speeds of 2400-3800 rpm and a 30% increase in microvial capacity.
Cat. No. 112011, Mini-BeadBeater-24, 115 volt
Cat. No. 112011EUR, Mini-Beadbeater-24, 230 volt
European buyers! The Mini-Beadbeater-24 is CE certified.
The Mini-Beadbeater-96 is high-energy, high-throughput cell disrupter. It can process one 2 ml deep well micro plate (96 samples), two 1 ml deep well microplates (192 samples) or, using a included 45 capacity polypropylene microvial rack, 0.6, 1.5 or 2 ml microvials. In a busy day thousands of samples can be processed.
Cat. No. 1001, Mini-Beadbeater-96, 115 volt.
Cat. No. 1001EUR, Mini-Beadbeater-96, 230 volt.
Beads and vials vary with the application and should be ordered separately. For selecting Beads and Vials see their links in Browse box in the left margin.
The Mini-Beadbeater-Plus™ is our upgraded version of the venerable Mini-BeadBeater-1. It is a high-energy single-vial bead-mill used to disrupt cells of biological samples. The Mini-BeadBeater-Plus can also grind samples of friable minerals or organic materials. Up tp 0.4 g of material is placed in a 2 ml microvial containing glass-, ceramic- or steel beads and "beadbeat" for 10 to 99 seconds. Typically, in a busy afternoon, about 20 samples can be processed for analysis. For much larger sample through-put, see our multi-vial MBB-16, MBB-24 and MBB-96 . Protocols developed using the Mini-BeadBeater-PLUS transfer to other Mini-BeadBeater models .
Cat. No. 1018, Mini-BeadBeater-Plus, 110/220 volt
Note: Required vials and beads vary with the particular sample being disrupted and should be purchased separately. For guidance on selecting Beads and Vials see the links in Browse box in the left margin of this web page. If you would like to discuss your application with a technical expert e-mail us or give us a call at 918-336-3363.
- Extremely Fast Cell Lysis...<20 sec
- Perfect for Small Samples
- 12 Vial Capacity
- Simple One Button Operation
The NEW SoniBeast™ high energy bead-mill cell disruptor completely disrupts small samples of tissue or cells in seconds rather than minutes. It holds up to twelve 0.6 ml PCR microtubes each containing 1-100 mg of sample, or six 2 ml microvials each containing 10-400 mg sample. The SoniBeast is designed for wet milling in water-based media. Common applications are the isolation of intracellular protein and nucleic acids.
We're really excited about this amazing new cell disrupter. It's the biggest improvement in bead-mill cell disrupter design since the beadbeater cell disruptor was introduced 30+ years ago.