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Selected Applications Using the Mini-Beadbeater

Extraction from Soil & Wastes

The Extraction and Purification of Microbial DNA from Sediments, Andrew Ogram et. al., J. Microbiol. Methods, Vol.7, p.57-66 (1987)

Methods for microbial DNA extraction from soil for PCR amplification, C. Yeates, et. al., Biological Procedures Online (1998)

Small-scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil, C.R. Kuske, et. al., Appl. Environ. Microbiology, Vol.64, p.2463-2472 (1998)

Evaluation and Optimiztion of DNA Extraction and Purification Procedures for Soil and Sediment Samples, D.N. Miller et. al., Appl. Environ. Microbiology, Vol.65, p.4715-4724 (1999). Click for web copy

Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-based Microbial Community Composition, R.I. Griffiths et. al., Appl. Environ. Microbiology, Vol 66, p.5488-5491(2000)

Simultaneous DNA/RNA Extraction from Soil, a protocol based on a modified method of Griffiths et. al.(2000), from Eoin Brodie, April 2003.  References a Fast-Prep machine.  Call for MBB equivalent speed settings.

Improved Extraction of PCR-quality Community DNA from Digesta and Fecal Samples, Zhongtang Yu and Mark Morrison, BioTechniques, Vol 36, p.808-813 (2004).   Content: Beadbeating gives "1.5 to 6 fold increase in DNA yield compared to other methods"

Comparitive analysis of fecal DNA extraction methods with phylogenetic microarray: Effective recovery of bacterial and archaeal DNA using mechnical cell lysis, Anne Solonen et al, J Microbiological Methods, Vol 81, p.127-134 (2010) Content:  Four widely used methods are compared.  DNA yields varied up to 35 fold.  A modified protocol of Yu and Morrison was the best.

A  Methods Protocol for the Extraction of DNA from Sediment/Soil Samples

Virus & Spores

West Nile virus Epidemic in Horses, Tuscany Region, Italy, Gian L Autorino et. al., Emerg. Infect. Dis., Vol.8, No..12, (2002) (serial online),

Reexamination of the Role of Autolysis in the Development of Myxococcus xanthus, Kathleen A. O'Connor and David R. Zusman, J. Bacteriology, Vol.170, p.4103-4112 (1988)

A Field Investigation of Bacillus anthracis Contamination of USDA and Other Washington, DC Buildings during the Antrax Attack of October 2001, James A. Higgins, et. al., Appl. Environ. Microbiology, Vol.69, p.593-599 (2003)

Invertebrates

Disruption of the Nematode Worm C. elegans, A procedural note, Christopher Hopkins, (June 2005). (Click for web copy)

Hair

Simultaneous Quantification of Opiates, Cocaine, and Metabolites in Hair by LC-APCI-MS/MS, Karl B. Scheidweiler and Marilyn A. Huestis, Anal. Chem., Vol.76 (15), p.4358–4363 (2004)

Pathogens in Plants

Comparison of Tissue-Disruption Methods for PCR-Based Detection of Plant Pathogens, Paul Vincelli and Bernadette Amsden, Plant Disease, Vol.97, No.3, p.363-368 (2013)